Isothiocyanate from Broccoli, Sulforaphane, and Its Properties

extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties

آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...

Studies on statistical optimization of sulforaphane production from broccoli seed

Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major factors (hydrolysis time, water volume and ethyl acetate volume) were screened out through Plack...

Gas Chromatography/Mass Spectrometry Method for the Determination of Sulforaphane and Sulforaphane Nitrile in Broccoli

Phytochemicals, especially the secondary metabolites synthesized by plants, play key roles in human nutrition, health, wellness, and disease prevention. Some phytochemicals may be harmful to human health. For example, two closely related 4-methylsulfinylbutyl glucosinolate [glucoraphanin] hydrolysis products from broccoli (Brassica oleracea L. var. Botrytis), 1-isothiocyanato-4-(methylsulfinyl)...

Isothiocyanate and D,L-Sulforaphane on Differential Effects of Phenethyl

Sulforaphane, a dietary component of broccoli/broccoli sprouts, inhibits breast cancer stem cells.

PURPOSE The existence of cancer stem cells (CSCs) in breast cancer has profound implications for cancer prevention. In this study, we evaluated sulforaphane, a natural compound derived from broccoli/broccoli sprouts, for its efficacy to inhibit breast CSCs and its potential mechanism. EXPERIMENTAL DESIGN Aldefluor assay and mammosphere formation assay were used to evaluate the effect of sulfo...